Journal: Molecular immunology

Article Title: Complement activation on neutrophils initiates endothelial adhesion and extravasation

doi: 10.1016/j.molimm.2019.09.011

Figure Lengend Snippet: Anti-GPI Ab recognizes an antigen on neutrophil cell surface. A) Peripheral white blood cells were stained for GPI (red) and Gr-1 (green, neutrophils), F4/80 (green, monocytes), CD19 (green, B cells), or CD3 (green, T cells). Colocalization appears yellow (see also Figure S6). Scale bar = 10 μm. B) Whole blood was stimulated with normal serum or HI K/BxN serum. Red blood cells were lysed and white blood cells were stained for MPO (green) and mouse IgG (red). DAPI stains nuclei blue. Scale bar = 5 μm.

Article Snippet: Cytospin samples were blocked with the anti-FcR mAb (1:100 dilution, cat#101302, BioLegend) in 5% BSA in PBS buffer for 1 hour at RTand probed with anti-mouse IgG (1:100 dilution, cat#115-295-164, Jackson ImmunoReseach) and biotinylated anti-mouse myeloperoxidase (MPO, 1:100 dilution, cat# {"type":"entrez-protein","attrs":{"text":"S22223","term_id":"95048","term_text":"pir||S22223"}} S22223 , Hycult Biotech) and for 2 hours at RT followed by streptavidin Alexa Fluor 488.

Techniques: Staining